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Pathogenic human coronavirus infections: causes and consequences of cytokine storm and immunopathology

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Luciferin detection reagent (LDR) (100 l) was added to all wells, briefly shaken and incubated for 15 min at room temperature and the luminescence measured using microplate imager (BioTek Cytation3)

Supporting our cytochrome c assays, we confirmed no change in mitochondrial outer membrane permeabilization (MOMP) in cells surviving 5FU treatment (compared to the dimethyl sulfoxide group), and no increase in mitochondrial apoptotic priming based on BH3-profiling 15,16 induced by 5FU treatment (Extended Data Fig

But do not assume B is in the mix unless explicitly stated