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Despite their effectiveness, they face limitations such as high costs, complex data interpretation, sensitivity and specificity issues, limited spatial resolution, and restricted coverage to known CpG sites, offering limited insights into the underlying mechanisms of methylation (57)

found that the aucubin iridoid agnuside from chaste tree extract significantly inhibited iron II-mediated free radical oxidation of deoxyribose ( P

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The column was then washed with 20 CV of DPBS/0.05% (v/v) sodium azide, followed by elution with 9 CV of 2 M MgCl 2 (Sigma-Aldrich)/20 mM Tris-HCl (Sigma-Aldrich) buffer (pH 7.4) at a flow rate of 2 mL/min