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The OD 600 value and methanol concentration of the culture were monitored through manual sampling

L-NAME and L-arginine were commercially purchased (Sigma, United States)

The structure of Th-2-31 was confirmed by NMR: 1 H NMR (400 MHz, Chloroform-d) 7.81 (d, J = 1.7 Hz, 1H), 6.86 (d, J = 1.9 Hz, 1H), 4.043.78 (m, 1H), 3.19 (td, J = 10.0, 4.2 Hz, 1H), 2.44 (s, 3H), 2.01 (d, J = 10.6 Hz, 4H), 1.89 1.64 (m, 4H), 1.58 (d, J = 13.0 Hz, 1H), 1.47 1.01 (m, 9H)

Neuro Endocrinol Lett 41(1):19 Al-Jowari SA-K (2024) Physiological impacts of cauliflower in hyperthyroid rabbits