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Milk Thistle: Active Compounds and Human Trial Data Milk thistle (Silybum marianum) seeds contain silymarin, a flavonolignan complex comprising silybin, silydianin, and silychristin

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Methods An extensive literature review was conducted through the online databases PubMed, Science Direct, and Springer Link up to August 2025, using the keywords cyclophosphamide, acrolein, ovarian failure, premature ovarian insufficiency/POI, oxidative stress, fertility preservation, cancer, myeloperoxidase/MPO, glutathione/GSH, superoxide dismutase/SOD, reactive oxygen species/ROS, and antioxidants

The gradient started at 5% B (held for 2.5 min) and was linearly increased to 30% B within 21.5 min, to 50% B within 2 min, then to 85% B within 0.5 min (held for 2 min)