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Additionally, Wiss and LaFata (88) found that UPF was associated with reduced leptin levels, hedonic eating, and decreased satiety

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The eluted proteins were buffer-exchanged to DPBS and concentrated using Amicon Ultra-15 Centrifugation Filter Units (Millipore) with a 30 kDa cutoff by centrifugation at (290400) g at 4 C

Carotenoids are natural pigments present in several plants that act as stabilizers of unstable molecules due to their ability to reversibly chelate reactive oxygen species and other free radicals, absorbing the energy of these molecules and dissipating it in the form of heat

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