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To activate the non-canonical inflammasome, peritoneal macrophages were stimulated with LPS (100 ng/mL) for 3 hr followed by intracellular LPS delivery (1 g/mL) by transfection using lipofectamine 2000 (Invitrogen, 11668027), according to the manufacturers protocol, in OptiMEM (Gibco, 31985-070) for 16hr

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In the absence of GPR146, impaired adipocyte differentiation and reduced lipolytic capacity together lead to diminished efflux of free fatty acids (FFAs) from adipose tissue

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