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The more you smoke, the more likely you are to notice tooth discoloration quickly

Samples were collected 24 hours post-transfection for RNA analysis and 48 hours for protein analysis

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Pun-Based Title: Meaning, of course, both "The importance of being named Ernest" and "the importance of being sincere." The Reveal: Jack's true parentage is revealed before the end of the play, and of course instantly resolves everything