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In the laboratory of allergic diseases 2 (LAD2) human mast cell line, quercetin reduced Substance P- and C48/80 (a compound used to promote histamine release from mast cells in experiments)-induced calcium flux in a dose-dependent manner, with concentrations above 100 M demonstrating effects comparable to dexamethasone at 100 M

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IC50 was calculated according to cell viability using the nonlinear regression analyses in GraphPad Prism 10.0 software