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This further supports why a higher drug concentration is needed at 48 h to achieve the same cytotoxic effect, considering 4T1 cells short doubling time of 13.6 h (Simoes et al

In that context, Cys-DIA was used to analyze Cys-containing peptides purified with Thiopropyl Sepharose TM 6B beads (as discussed in section Thiol-affinity resin-assisted capture) to increase Cys-proteome coverage up to 35% compared to conventional DIA/SWATH approach

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