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found that FTA not only restored the disrupted structure of intestinal microbiota, specifically, Ace index increased significantly, Shannon index decreased significantly, Lachnospiraceae_NK4A136_group, Ruminococcaceae_UCG-014, LactobacillusBacteroides, Prevotellaceae_UCG-001, Helicobacter and Alloprevotellabut were adjusted to benefit the health of the host, also promoted the production of SCFA (particularly butyric acid)

The medium was exchanged with 1.5% GelTrex in mTeSR1 (Day-2), mTeSR1 (Day-1), RPMI (Thermo Fisher Scientific) + 12 M CHIR99021 (Tocris Bioscience, Bristol, UK) (Day 0), or RPMI + B27 supplement (Thermo Fisher Scientific) (Day-1.5), and the cells were fed every 23 days to promote kidney organoid differentiation

cytokine array was used, showing that several molecules involved in T helper 1 (Th1) (pro-inflammatory) and/or Th2 (more protective) polarization, were increased in PBMC from AMN patients ( Fig

doi:10.1021/acsnano.2c04886 178

Lancet 2023;402:72030.ArticlePubMed 105