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Bibcode:2016Natur.532..255J

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Using a coupled assay with Cp1A and Cp1B to form t -ES-l-Ile (vide infra), the stereochemistry of the epoxide formed by Cp1A was assigned to be (2 S ,3 S ) on the basis of a comparison of retention times to those of synthetic (2 S ,3 S )- t -ES-l-Ile ( 14 ) and (2 R ,3 R )- t -ES-l-Ile (Extended Data Fig