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Western blot analyses were performed on nitrocellulose membranes after blocking in TBS-T containing 5% milk, using the anti-BAX antibody (clone 2D2, Cell Signaling Technologies, 1:1000) and the anti-cytochrome c antibody (clone 7H8.2C12, Abcam, 1:1000 dilution, ab13575) and incubating overnight at 4 C

Gawish RA, Samy EM, Aziz MM

Making sure your embryos are safe and sound throughout the entire IVF cycle

This adjustment is justified by the evidence that these mutations, typically associated with AML evolving from an antecedent hematologic disease, can also be detected in de novo AML and confer adverse risk even in the absence of MR morphological features and/or a prior history of MDS

Nucleic Acids Res 48 , 28532865 (2020)