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10.3390/ijms22020614 7 BoseC.HindleA.LeeJ.KopelJ.TonkS.PaladeP

Selective toxicity of neonicotinoids attributed to specificity of insect and mammalian nicotinic receptors

Pull-down assay Five micrograms of the bait protein c-MET927-TSI3-His was immobilized to TSE resin by incubating for 30 min on ice and washed with binding buffer PBS (Gibco) supplemented with10 M Tinzaparin and 2 mM CaCl2