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However in our small cohort, GPX3 levels were neither significantly associated with a particular ILD nor correlated with lung function, as proposed by the animal study

PCR assays PCR assays for confirmation of the identified bacteria and determination of antibiotic resistance genes Since polymerase chain reaction (PCR) and quantitative PCR (qPCR) are very sensitive for detecting total DNA present in a sample, including DNA from live and dead cells 19 and due to process-related DNA changes, PCR-based approaches allow the direct identification of DNA damage during disinfection 31

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While gene therapy is available for a small subset of patients with specific mutations, developing a therapeutic that broadly targets the cellular stresses that lead to cell death could address a major unmet need

Bacterial cells were collected and lysed in STE buffer (10 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM EDTA) containing 0.1% Triton X-100, 100 g/mL lysozyme, 1 mM PMSF, and 1X PIC