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Next, the plants were treated with 200 M cycloheximide and total proteins were isolated at 0, 3 and 6 h from GSNO treated and non Cl pH 7.5, 150 mM NaCl, 0.5 % Triton_X_100 (v/v) and protease inhibitor, Thermo Scientific) at 4 C and centrifuged at 13,000 rpm for 30 min at 4C

doi: 10.3390/ijms25115874 159

In BV-2 cells treated with fluoride, SOD levels decreased, while those of MDA, ROS, superoxide anions (O 2 ), and NOS significantly increased in a dose-dependent manner (Shuhua et al., 2012)

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