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The hypothalamic-pituitary-adrenal and the GH/IGF-I axis are also closely associated, as cortisol is required for physiological GH secretion

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GHR is widely expressed in GH target cells, which can combine with GH to activate diverse signal cascades, including mitogenic signaling through Janus kinase (JAKs) signal transducers and activators of transcription (STATs) (Vanderkuur et al., 1997), phosphoinositide-3-kinase (PI3K)/Protein kinase B (PKB or AKT)/mammalian target of rapamycin (mTOR) pathways (Hayashi and Proud, 2007) and phospholipase C (PLC)/Protein kinase C (PKC) ( GHR will further disrupt the normal development of the organism and lead to dwarfism phenotype in a wide array of species (Lin et al., 2018)

A repeated-measures two-way ANOVA revealed a significant main effect of the factors treatment (F(1,16)=12.47, P=0.0028), time (days) (F(5,80)=4.437, P=0.0012), and a significant treatment x time interaction (F(5,80)=10.89, P 0.05;FIG

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