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To directly assess the role of BMAL1 in AD-related pathology, we engineered an adeno-associated virus serotype 2/5 (AAV2/5) carrying a short hairpin RNA targeting Bmal1 (AAV2/5-ShRNA ( Bmal1 )), designed to specifically target ChP epithelial cells [21]

1 H NMR (400 MHz, DMSO-d6) = 10.49 (s, 1H), 8.67 (dd, J = 5.2, 1.8 Hz, 1H), 8.00 (td, J = 7.7, 1.9 Hz, 1H), 7.92 7.83 (m, 2H), 7.66 (d, J = 8.1 Hz, 1H), 7.51 (ddd, J = 7.3, 4.8, 1.1 Hz, 1H), 7.06 6.89 (m, 2H)

Figure 2 To demonstrate that the anticancer response to LDL-DHA was not a murine specific effect we evaluated the well differentiated human HCC cell line HUH7 ( Supplementary Figure 2 )

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Alternatively, you can use cell-free expression systems for protein expression