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Using this strategy, we obtained oligodendroglial transcriptional signatures (Supplementary Data 1 and Supplementary Table 3) enriched either in progenitor cells (1898 genes) or in more mature stages (2099 genes), which we refined to 3372 genes (Supplementary Data 1 and Supplementary Table 4) using further selection criteria (Methods)

Cells were collected after 72 h and lysed by gentle nutation in wash buffer (50 mM HEPES, 150 mM NaCl, 2.5 mM MgCl 2 , 1 mM TCEP, pH 7.4) supplemented with 0.4% CHAPS and Protease Inhibitor (Roche) for 1 h

For example, based on recent published data, one could envision that treatment of ex vivo hybrid Th1/17 cells with these inhibitors could increase their tumor suppressive effects [50]