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Briefly, cell extracts were prepared using the RIPA lysis buffer (150 mM sodium chloride, 1% NP-40, 0.1% SDS, 50 mM Tris, pH 7.4) containing 1 mM -glycerophosphate, 2.5 mM sodium pyrophosphate, 1 mM sodium fluoride, 1 mM sodium orthovanadate, and a protease inhibitor (Roche, Cat

For the subparticles of C34 Fab:VLP complex around 5 f axis, duplicate particles were removed around 5f axis, duplicate particles were removed, and particles underwent no-alignment 3D classification with a denovo mask centered at the 5 Fab molecules around the 5f axis with a 95 radius

10.1007/s00125-011-2342-y

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It is a structured clinical sequence built on published evidence, adapted to the specific skin biology of our patient population, and refined through practice