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The percentage of SA--gal staining-positive cells was calculated in at least 3 random fields

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21 In mice, pre-treatment with n-acetylcysteine prevents toxicity from even a very high acetaminophen dose

The supernatant was then combined with 2.7 ml phosphate buffer (0.1 M, pH 7.4) and 0.2 ml 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and incubated for 5 min